Determination of Biological Oxidative Stress Using High Performance Liquid Chromatography with Electrochemical Detection (HPLC-ECD)

✍ Scribed by Hensley, Kenneth ;Williamson, Kelly S. ;Maidt, Michael L. ;Prasad Gabbita, S. ;Grammas, Paula ;Floyd, Robert A.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 146 KB
• Volume: 22
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/(sici)1521-4168(19990801)22:8<429::aid-jhrc429>3.0.co;2-s

✦ Synopsis

For three decades, high performance liquid chromatography has proven itself to be a powerful, flexible, and inexpensive tool for basic and clinical research. Recent advances in our understanding of disease have prompted a demand for more sensitive and selective methods of routine bioanalysis, particularly with respect to the determination of oxidative metabolites and biomarkers of oxidative stress. Multidimensional detectors utilizing coulometric arrays offer a solution to these research needs. The challenge to the bioanalyst is now to creatively apply HPLC-ECD technology to promising research and clinical enterprises.