Quantification of 9-Carboxy-11-nor-Δ9-tetrahydrocannabinol in Urine Using Brominated 9-Carboxy-11-nor-Δ9-tetrahydrocannabinol as the Internal Standard and High-Performance Liquid Chromatography with Electrochemical Detection
✍ Scribed by Daniel H. Fisher; Marc I. Broudy; L. Megan Fisher
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 459 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
A method was developed for quantitating 9-carboxy-ll-nor-A'-tetrahydrocannabinol in human urine as part of the process for validating an automated enzyme immunoassay for marijuana metabolites. Sample cleanup was accomplished using a mixed-mode solid-phase extraction. 9-Carboxy-ll-nor-A'-tetrahydrocannabinol and the internal standard, brominated 9-carboxy-ll-nor-A9-tetrahydrocannabinol, were quantified using high-performance liquid chromatography with electrochemical detection (+0.85 V). The linear range for this method is 0.0124.20 pglmL. No interference was seen for 22 drugs and metabolites. The pooled relative standard deviation is 4.1% (n=27) for the quality control samples. This method was compared to gas chromatography with mass spectrometry by linear regression analysis. The slope of the line is 1.00*0.05 (standard error), the intercept is approximately zero, the coefficient of determination is 0.994, and the standard error of the estimate is 0.006 pglmL.