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Determination of bile acids and lysolecithin in artificial and natural gastric juice by HPLC for characterization of antacida

✍ Scribed by Nieder, M. ;Jaeger, H. ;Guthauser, U. ;Althaus, B.

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
473 KB
Volume
8
Category
Article
ISSN
0935-6304

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✦ Synopsis

Work

-up procedures and HPLC separation systems for determination of taurocholic, glycocholic, chenodeoxycholic, and cholic acids and lysolecithin in artificial and natural gastric juice are described. These compounds are used for testing the binding capacity of antacida to the individual analytes. Work-up is simple, no extraction or filtration being required. The optimized combinations of stationary and mobile phases allow selective and sensitive determination of the respective bile acids in gastric juice. For optimization, the capacity factors of two related bile acids were evaluated for numerous commercial stationary phases. The mechanism of retention is different for free and conjugated bile acids. Special aspects of routine analysis are discussed.

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