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Determination of benzalkonium chloride in aqueous ophthalmic preparations by high-performance liquid chromatography

✍ Scribed by A. Gomez-Gomar; M.M. Gonzalez-Aubert; J. Garces-Torrents; J. Costa-Segarra

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
288 KB
Volume
8
Category
Article
ISSN
0731-7085

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✦ Synopsis

A high-performance liquid chromatographic method is described for the determination of homologues of benzalkonium chloride in aqueous ophthalmic preparations. The technique involves direct injection of the sample on a 5-microns Spherisorb-CN column. The mobile phase is acetonitrile-triethylamine (0.1%, v/v) in water (pH 2.5; 40:60, v/v). Detection is carried out at 215 nm. The method is rapid, specific, reproducible and simple, and is especially useful for the assay of this preservative in stability studies and quality control procedures.

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