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Determination of association constants of dansyl-amino acids and β-cyclodextrin in N-methylformamide by capillary electrophoresis

✍ Scribed by Dr. István E. Valkó; Heli Sirén; Marja-Liisa Riekkola

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
424 KB
Volume
18
Category
Article
ISSN
0173-0835

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✦ Synopsis

Determination of association constants of dansyl-amino acids and fl-cyclodextrin in N-methylformamide by capillary electrophoresis

The use of nonaqueous background electrolytes in capillary electrophoresis (CE) is a promising new trend which should widen the scope of this technique. We demonstrate the chiral separation of dansyl-amino acids (Dns-AAs) in N-methylformamide (NMF) using B-cyclodextrin (B-CD) as chiral selector. The solubility of B-CD is much better in NMF than in water, allowing high concentration of the chiral selector and successful enantioseparation despite the weak host-guest interaction between the Dns-AAs and B-CD. The association constants for the complexation between Dns-AAs and B-CD could be calculated from the electrophoretic mobilities, with attention paid to the change in viscosity of the electrolyte upon addition of B-CD. The association constants ranged between 2 and 13 M-'.

📜 SIMILAR VOLUMES

Chiral separation of dansyl amino acids
Chiral separation of dansyl amino acids in capillary electrophoresis using mono-(3-methyl-imidazolium)-β-cyclodextrin chloride as selector
✍ Weihua Tang; Teng Teng Ong; Siu-Choon Ng 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 567 KB

## Abstract Enantioseparations of fourteen dansyl amino acids were achieved by using a positively‐charged single‐isomer β‐cyclodextrin, mono‐(3‐methyl‐imidazolium)‐β‐cyclodextrin chloride, as a chiral selector. Separation parameters such as buffer pH, selector concentration, separation temperature,