Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis

✍ Scribed by Makoto Tsunoda; Masaru Kato; Takeshi Fukushima; Tomofumi Santa; Hiroshi Homma; Hiroko Yanai; Tomoyoshi Soga; Kazuhiro Imai

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 68 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199908)13:5<335::aid-bmc868>3.0.co;2-4

✦ Synopsis

Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3, 6-tri-O-methyl)-beta-cyclodextrin(TM-beta-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-beta-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 nM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.