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Determination of ascorbic acid in nanolitre samples by means of capillary batch injection analysis

✍ Scribed by U. Backofen; F. -M. Matysik; W. Hoffmann; H. -J. Ache

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
79 KB
Volume
12
Category
Article
ISSN
0269-3879

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✦ Synopsis

Capillary batch injection analysis (CBIA) is applied to the determination of ascorbic acid in nanolitre samples at neutral pH. It is demonstrated that pulsed amperometric detection is superior to constantpotential amperometric detection with respect to signal stability. The calibration plot is linear within the studied concentration range between 0.5 mM and 5 mM and the reproducibility is characterized by a relative standard deviation of 0.80% for 10 successive injections of 188 nL of 3.5 mM ascorbic acid. The attainable sample throughput is up to 500 per hour.

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