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Determination of apomorphine in canine plasma by liquid chromatography–electrospray ionization mass spectrometry and its application to a pharmacokinetic study

✍ Scribed by Bei Yang; Yunqiu Yu; Lei Cai; Chunhui Deng; Gengli Duan

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
484 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Determination of apomorphine in canine plasma by liquid chromatography-electrospray ionization mass spectrometry and its application to a pharmacokinetic study An LC-ESI-MS method was developed and validated for the assay of apomorphine in canine plasma using one-step liquid -liquid extraction. The analytes were separated on a Phenomenex Gemini C 18 (150 mm62.0 mm id 3 lm) column and determined by MS in the positive ion mode. The linear range was 0.4 -40 ng/mL with an LOD of 0.2 ng/mL for apomorphine in plasma. The intraday and interday precision and accuracy of quality control samples were a 5.9% RSD and a 7.5% bias for apomorphine. Extraction recoveries were A 80%. The validated method was successfully applied to analyze canine plasma samples in a pharmacokinetic study of apomorphine in dogs and detailed pharmacokinetic parameters were calculated.

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