Determination of Active Ingredients of Polygonum cuspidatum Sied. et Zucc. by Capillary Electrophoresis with Electrochemical Detection

✍ Scribed by Qingcui Chu; Youyuan Peng; Jiannong Ye

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 125 KB
• Volume: 16
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200302968

✦ Synopsis

Abstract

High‐performance capillary electrophoresis with electrochemical detection (CE‐ED) was employed to analyze active ingredients of Polygonum cuspidatum Sied. et Zucc. in crude herbs and compound medicines. trans‐Resveratrol, emodin, chrysophanol, and rhein are major important active ingredients. Operated in a wall‐jet configuration, a 300 μm diameter carbon‐disk electrode was used as the working electrode, which exhibits good response at +950 mV (vs. SCE) for the four analytes. Under the optimum conditions, these analytes were base‐line separated within 27 min in a borax buffer (pH 8.7). Notably, excellent linearity was obtained over two orders of magnitude with detec tion limit (S/N=3) ranged from 4.2×10^−7^ to 5×10^−8^g mL^−1^ for all analytes. This method was successfully used in the analysis of Polygonum cuspidatum Sied. et Zucc. and Jindan tablets with relatively simple extraction procedures, and the assay results were satisfactory.