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Determination of acenocoumarol in plasma and urine by double radioisotope derivative analysis

✍ Scribed by Y. Le Roux; J. Richard

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
448 KB
Volume
66
Category
Article
ISSN
0022-3549

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✦ Synopsis

Acenocoumarol, to which a 14C-labeled internal standard has been added, is extracted at pH 4 into ethyl acetate-heptane (20:80 v/v), back-extracted into aqueous sodium hydroxide solution after solvent washing with a pH 7 buffer, and reextracted after acidification in the solvent mixture. It is then acetylated with 3H-acetic anhydride. The acenocoumarol acetyl derivative is separated from the metabolite derivatives by TLC, and its radioactivity is measured. The method is specific and sensitive to a concentration of 8 ng/ml.

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