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Spectrofluorimetric determination of nomifensine in human plasma and urine by derivatization with fluorescamine

✍ Scribed by Sevgi Tatar Ulu

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
174 KB
Volume
27
Category
Article
ISSN
1522-7235

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✦ Synopsis

ABSTRACT

A sensitive, simple and rapid spectrofluorimetric method was developed for the determination of nomifensine in human plasma and urine. The present method was based on the derivatization by fluorescamine in phosphate buffer at pH 4.0 to produce a highly fluorescent product which was measured at 488 nm (excitation at 339 nm). The method was validated according to the ICH guidelines with respect to linearity, limit of detection, limit of quantification, accuracy, precision, recovery and robustness. The assay was linear over the concentration ranges 100–2,000 and 50–2,000 ng/mL for plasma and urine, respectively. The limits of detection were calculated to be 13.9 and 7.5 ng/mL for plasma and urine, respectively. The method was successfully applied to the analysis of the drug in human plasma and urine. Copyright Β© 2011 John Wiley & Sons, Ltd.

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