An HPLC (high performance liquid chromatography) method with laser induced fluorescence (LIF) detection is described for the determination of 4-hydroxy-2-nonenal (HNE) formed from lipid peroxidation in rat hepatocytes. Carbonyl compounds were fluorescently labelled by incubating the hepatocyte sampl
β¦ LIBER β¦
Determination of 4-hydroxy-2-nonenal by precolumn derivatization and liquid chromatography with laser fluorescence detection
β Scribed by Yi-Ming Liu; Jian-Rong Miao; Toshimasa Toyo'oka
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 471 KB
- Volume
- 719
- Category
- Article
- ISSN
- 1873-3778
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## Abstract A rapid and sensitive HPLC enantioselective method with fluorescence detection was developed to determine (β)β(__R__) and (+)β(__S__) enantiomers of the metabolites of citalopram, demethylβ and didemethylβcitalopram in plasma and brain tissue. This assay involves preβcolumn chiral deriv