Determination of 3,4-dihydroxyphenylethylene glycol and 3,4-dihydroxyphenylacetic acid in blood plasma by liquid chromatography with amperometric detection

✍ Scribed by Britt-Marie Eriksson; Stina Gustafsson; Bengt-Arne Persson

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 577 KB
• Volume: 2
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(84)80081-3

✦ Synopsis

Liquid chromatography with amperometric detection was employed for the determination of the dihydroxycatecholamine metabolites, 3,4-dihydroxyphenylethylene glycol (DOPEG) and 3,4_dihydroxyphenylacetic acid (DOPAC) in plasma. The compounds were isolated from plasma by adsorption onto alumina, the elution from which was found to be strongly dependent on the acid used. Conditions for the separation on octadecyl-bonded silica were evaluated with particular reference to the influence of pH, ion-pairing anions and quaternary ammonium ions. Recoveries of ca 85% and relative standard deviations of about 3% were obtained for the assay of endogenous concentrations.