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Determination of 2-pyrrolidone-5-carboxylic and α-ketoglutaramic acids in human cerebrospinal fluid by gas chromatography

✍ Scribed by Arthur J.L. Cooper; Amiya K. Dhar; Henn Kutt; Thomas E. Duffy

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
675 KB
Volume
103
Category
Article
ISSN
0003-2697

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✦ Synopsis

A gas chromatographic method is described for the quantitation of some substituted 2-pyrrolidones of biological interest. These compounds are converted to the trimethylsilylated derivatives with N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA). The derivatized pyrrolidones are well separated by gas chromatography, and flame ionization provides a sensitive technique for their detection. The method was applied to the simultaneous estimation of 2-pyrrolidone-S-carboxylic acid and 5-hydroxy-2-pyrrolidone-5carboxylic acid (the cyclic lactam and predominant form of cr-ketoglutaramic acid) in human cerebrospinal fluid. Both compounds were found to be increased in cerebrospinal fluid of patients with hepatic encephalopathy. It is suggested that the increases are secondary to elevated amino acids in cerebrospinal fluid and plasma.

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Determination of amino acids in human ce
Determination of amino acids in human cerebrospinal fluid by gas chromatography
✍ Labadarios, D. ;Moodie, I. M. ;Hough, B. J. ;Burger, J. A. 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 384 KB

In the present study, the use of gas chromatography (GC) for the determination of amino acids in human cerebrospinal fluid (CSF) is described. Although some amino acids may be determined using a packed column following the removal of glucose, the major interfering component, the inadequate resolutio