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Determination of 14C-labeled plasma l+α-alanine specific radioactivity

✍ Scribed by P.E.B. Reilly

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
468 KB
Volume
65
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described which anables the specific radioactivity of W-labeled L + a-alanine in plasma to be determined. Plasma alanine concentration is determined spec~ophotometrically using alanine dehydrogenase. In a separate procedure, this enzyme is also used to convert '*C labeled alanine and added carrier alanine, to pyruvate. The phenylhyd~zone derivative of the pyruvate is then prepared and is assayed for radioactivity after crystallization to constant specific radioactivity. A maximum error of 1.5% for any one specific radioactivity determination was found.

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