Determination of 1-nitropyrene by thin-layer chromatography in a diesel exhaust particulate extract

✍ Scribed by Brown, K. K. ;Poole, C. F.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 413 KB
• Volume: 7
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240070905

✦ Synopsis

Abstract

A method is described for the determination of 1‐nitropyrene in a diesel exhaust particulate extract by thin‐layer chromatography. 1‐Nitropyrene is determined as 1‐aminopyrene after prechromatographic reduction and isolation by solvent partition. The fluorescence response of 1‐aminopyrene is approximately 1000‐fold greater than that of 1‐nitropyrene. The poor fluorescence stability of 1‐aminopyrene on silica gel plates prevents in situ detection by scanning densitometry unless the plates are impregnated with a mixture of 2,6‐di‐tert‐butyl‐4‐methylphenol and Fomblin H‐Vac. A linear response from 1 to 150 ng of 1‐aminopyrene and a detection limit of 1.0 ng were obtained using the impregnated plates. For fourteen samples of a diesel exhaust particulate extract the concentration of 1‐nitropyrene was determined to be 165 μg/g with an RSD = 9.0%. No significant interferences were observed from other nitro‐ or aminopolycyclic aromatic compounds present in the extract.