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Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings

โœ Scribed by Thomas J. BACH; David H. ROGERS; Harry RUDNEY

Book ID
115124299
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
980 KB
Volume
154
Category
Article
ISSN
1432-1327

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โœ Zafarul H. Beg; John A. Stonik; H.Bryan Brewer Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 312 KB

A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2