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Detection of water-soluble vitamins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using porphyrin matrices

✍ Scribed by Yi-Ting Chen; Yong-Chien Ling

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
319 KB
Volume
37
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The detection of water‐soluble vitamins B~1~, B~2~, B~6~, B~12~ and C by matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight mass spectrometry (TOFMS) was attempted by studying 17 porphyrin matrices. Comparative studies of porphyrin matrices, useful mass spectral window, matrix/analyte molar ratio (M/A), ultraviolet–visible absorption characteristics and quantitative results were made. Most porphyrin matrices provide a useful mass spectral window in the low‐mass range. The optimal M/A increases with increasing molecular mass of the vitamin. Vitamin B~12~ possesses the highest molecular mass and requires a higher M/A. The presence of hydroxyl or carboxyl groups in the porphyrin is an indicator of a useful MALDI matrix. Vitamins B~2~ and B~6~ were readily ionized upon irradiation with a 337 nm laser without the use of any porphyrin matrix. Improved linearity and sensitivity of the calibration curves were obtained with samples prepared with a constant M/A. The limits of detection and quantitation are at the picomole level. The results indicate that MALDI‐TOFMS with porphyrin matrices is a rapid and viable technique for the detection of low molecular mass water‐soluble vitamins. Copyright © 2002 John Wiley & Sons, Ltd.

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