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Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR

โœ Scribed by L. Wang; Y. Li; A. Mustapha


Book ID
108840572
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
627 KB
Volume
107
Category
Article
ISSN
1364-5072

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t