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Detection of tissue-specific effects by methotrexate on differentiating mouse embryonic stem cells

✍ Scribed by Cristian Pellizzer; Ezia Bello; Sarah Adler; Thomas Hartung; Susanne Bremer


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
243 KB
Volume
71
Category
Article
ISSN
1542-9733

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Most embryonic stem (ES) cell research is performed with a gas phase oxygen partial pressure (pO 2 ) of 142 mmHg, whereas embryonic cells in early development are exposed to pO 2 values of 0-30 mmHg. To understand effects of these differences, we studied murine ES (mES) growth, maintenance of stem c