Detection of telomerase activity in breast masses by fine-needle aspiration
โ Scribed by A. Scott Pearson; Lauren S. Gollahon; Nancy C. O'Neal; Hossein Saboorian; Jerry W. Shay; Thomas J. Fahey
- Publisher
- Springer
- Year
- 1998
- Tongue
- English
- Weight
- 796 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1068-9265
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## BACKGROUND: One of the limitations preventing the widespread use of fine-needle aspiration (FNA) is that it requires skill to obtain an adequate sample and well prepared smears. In this study, a new monolayer technique, the Cyto-Tek Mono-Prep (MP) system, which obviates the need for smear prepa
## Background: Fine-needle aspiration (fna) biopsy of palpable breast masses along with clinical and radiologic findings can provide rapid distinction between benign and malignant lesions. a preoperative determination of invasive or in situ carcinoma assists in the planning of definitive treatment.
We analysed telomerase activity in normal, benign and malignant breast tissues and in fine needle aspirates by a PCR-based assay. The tissue samples we used in this assay consisted of 20 cryostat sections, I0 pm thick, from each breast biopsy. This method was used to obtain effective extraction from