Telomerase activity in human breast cancer and benign breast lesions: Diagnostic applications in clinical specimens, including fine needle aspirates

✍ Scribed by Takashi Sugino; Kazuhiro Yoshida; John Bolodeoku; Hidetoshi Tahara; Ian Buley; Sanjiv Manek; Clive Wells; Steven Goodison; Toshinori Ide; Toshimitsu Suzuki; Eiichi Tahara; David Tarin

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 697 KB
• Volume: 69
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19960822)69:4<301::aid-ijc11>3.0.co;2-8

✦ Synopsis

We analysed telomerase activity in normal, benign and malignant breast tissues and in fine needle aspirates by a PCR-based assay. The tissue samples we used in this assay consisted of 20 cryostat sections, I0 pm thick, from each breast biopsy. This method was used to obtain effective extraction from small samples and to confirm the histological identity of the specimen by microscopical examination of serial sections. Fifty-two of 7 I breast carcinomas were positive for telomerase activity, and the intensity of this was strong in most cases, whereas all 6 samples of normal breast tissue and 17 of fibrocystic disease were negative and only I of I 5 fibroadeno-