Detection of streptomycins in raw milk by an antibody-capture immunoassay

✍ Scribed by P. Hammer; H. Kirchhoff; G. Hahn

• Book ID: 102623556
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 327 KB
• Volume: 275
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80307-7

✦ Synopsis

An mdlrect competltlve enzyme-hnked nnmunosorbent assay camed out as an antdnxly-capture test was developed for the determmation of streptomycms III mdk The antIserum was raised m rabbits usmg streptomycm hnked to a bactenal protem as lmmunogen The antkxxlres were concentrated by threefold ammomum sulphate preclpltation and reacted equally Hnth streptomycm and dlhydrostreptomycm No other cross-reactlons were observed To perform the test muxotltre plates were coated overmght with streptomycm (1 mg ml-') The mdk to be exammed was slummed and treated mth oxabc acid Antierum and mdk samples were nuxed and mcubated for 1 h Dependmg on the amount of strept&ycm or dlhydrostreptomycm m the sample, more orjess ant&x$ molecules remam free to be bound to the streptomycm coat The sensltmty of the test IS better than 16 Kg kg-' Quant&ication IS possible up to 100 bg kg-' wthout sample ddutlon Keynxx& Enzymatic methods, Immunoassay, Antlblotax, An&x&capture test, Enzyme-lmked mmmnosorbent assay, MI& Streptomycms