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Detection of antibody to varicella-zoster virus by competitive and IgM-antibody capture immunoassay

✍ Scribed by R. S. Tedder; R. Bridget Lord; P. P. Mortimer

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 702 KB
Volume: 8
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890080203

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✦ Synopsis

Abstract

A simple, sensitive, and specific competitive solid phase immunoassay for antibody to varicella‐zoster virus (anti‐VZV) is described. The assay uses reagents that can easily be prepared and is sparing of viral antigen. Using a solid phase coated with sheep IgG from a serum raised against human μ‐Fc, the same reagents will accurately detect anti‐VZV IgM.

The competitive assay divided sera from children and adults into immune and nonimmune groups that closely correlated with a history of previous VZV illness. It was not affected by the presence or absence of antibody to other herpes viruses. The IgM antibody capture assay demonstrated the presence of anti‐VZV IgM in sera from patients with both varicella and zoster and gave negative results in patients with infections unrelated to VZV and in healthy blood donors.

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