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Detection of soluble peroxidases by direct plant tissue isoelectric focusing

✍ Scribed by Blanca Vioque; Antonio López; José M. Castellano; Miguel A. Albi; Agustín Vioque

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
226 KB
Volume
7
Category
Article
ISSN
0173-0835

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✦ Synopsis

Detection of soluble peroxidases by direct plant tissue isoelectric focusing

Rectangular sections of leaves. vacuum-infiltrated with distilled water or aaueous v solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases (free and ionically bound) were released from the tissue on isoelectric focusing with resultant characteristic zymograms for the different plant tissues.

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✍ Udo Schumacher; Peter Trudrung 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 937 KB

Application of cryostat sections directly onto mini ultrathin polyacrylamide isoelectric focusing gels allows an elution of proteins out of the sections into the gels under conventional focusing conditions. Protein bands representing alkaline phosphatases can easily be identified on nitrocellulose a