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Detection of single nucleotide polymorphisms by PCR conformation-difference gel electrophoresis

✍ Scribed by Zhu, Weifeng; Deng, Yan; Jie, Kemin; Luo, Daya; Liu, Zhuoqi; Yu, Lehan; Zeng, Erming; Wan, Fusheng


Book ID
121313688
Publisher
Springer Netherlands
Year
2012
Tongue
English
Weight
474 KB
Volume
35
Category
Article
ISSN
0141-5492

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Detection of single nucleotide polymorph
✍ Tomoaki Mitani; Atsushi Akane πŸ“‚ Article πŸ“… 2005 πŸ› Elsevier Science 🌐 English βš– 134 KB

A simple and rapid analysis system for single nucleotide polymorphisms (SNPs) was investigated for the FUT2 gene using the temperature gradient gel electrophoresis (TGGE) method. The 426-bp or 259-bp FUT2 fragments were amplified from heterozygous samples using primers, and the heteroduplex and homo