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Detection of short-chain α-hydroxyaldehydic compounds as pentafluorbenzyloxime derivatives in bovine liver

✍ Scribed by Angelika Loidl-Stahlhofen; Kerstin Hannemann; Gerhard Spiteller

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
447 KB
Volume
77
Category
Article
ISSN
0009-3084

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✦ Synopsis

Pentafluorbenzyloxime derivatization allows fast, gentle and unambiguous identification of ~-hydroxyaldehydic lipid peroxidation products via GC/MS in biological material. Even 1.5 g of a bovine liver sample is sufficient to detect short-chain 2-hydroxyalkanales resulting from cleavage reactions of dioxygenated fatty acids. Quantification is achieved after secondary derivatization with N-methyl-N-t-butyldimethylsilyltrifluoracetamide (M-t-BSTFA) by mass spectrometry using characteristic ion traces of the derivatives. In addition, the corresponding (n-1)-hydroxy-n-oxo acids, previously unknown in biological material, could be detected.

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