Detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization
✍ Scribed by Freymuth, François; Vabret, Astrid; Galateau-Salle, Françoise; Ferey, Janine; Eugene, Geneviève; Petitjean, Joelle; Gennetay, Evelyne; Brouard, Jacques; Jokik, Mikael; Duhamel, Jean-François; Guillois, Bernard
- Book ID
- 121453744
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 824 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0928-0197
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During the winter season of 1994/1995, nasopharyngeal aspirates and blood samples of neonates who were admitted to the Neonatal Intensive Care Unit (NICU) (group 1) and infants with respiratory tract disease (group 2) were examined prospectively for the presence of respiratory syncytial virus (RSV).
## Abstract The polymerase chain reaction (RNA‐PCR) was used for specific detection of respiratory syncytial virus (RSV) genomes in clinical specimens. A set of primers was selected from conserved regions of the 1Band N genes for detection of both subgroups. The primers were found to be RSV specifi