Detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness
β Scribed by Ann R. Falsey; Mary C. Criddle; Edward E. Walsh
- Book ID
- 116630848
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 83 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1386-6532
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During the winter season of 1994/1995, nasopharyngeal aspirates and blood samples of neonates who were admitted to the Neonatal Intensive Care Unit (NICU) (group 1) and infants with respiratory tract disease (group 2) were examined prospectively for the presence of respiratory syncytial virus (RSV).
## Abstract The polymerase chain reaction (RNAβPCR) was used for specific detection of respiratory syncytial virus (RSV) genomes in clinical specimens. A set of primers was selected from conserved regions of the 1Band N genes for detection of both subgroups. The primers were found to be RSV specifi