Detection of Immobilized Proteins on Nit
Detection of Immobilized Proteins on Nitrocellulose Membranes Using a Biotinylation-Dependent System
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RΓΌdiger Graf; Peter Friedl
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Article
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1999
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Elsevier Science
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English
β 210 KB
Proteins are biotinylated after immobilization on nitrocellulose sheets by reaction with a biotinyl-succinimide ester. The biotinyl residues are visualized by streptavidin-peroxidase-based detection systems either by deposition of a colored formazan dye or by enhanced chemiluminescence (ECL), the la