Charge-coupled device camera-based detection of fluorescence-labeled proteins immobilized on nitrocellulose membranes

Polyacrylamide gel electrophoresis of re

Polyacrylamide gel electrophoresis of reducing saccharides labeled with the fluorophore 2-aminoacridone: Subpicomolar detection using an imaging system based on a cooled charge-coupled device

✍ Peter Jackson 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 787 KB

Numerous monosaccharides and oligosaccharides were derivatized at their reducing end groups with the fluorophore 2-aminoacridone. The resulting fluorescent compounds were separated by PAGE using two different buffer systems. One of these, a Tris borate buffer, enabled all of the fluorescent sacchari