We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only
β¦ LIBER β¦
Detection of polymorphisms using thermal cycling with a single oligonucleotide on a DNA sequencing gel
β Scribed by Vincent Murray; Chutima Monchawin; Murray J. Cairns; Phillip R. England; Don Leigh; Brian L. McDonald
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 449 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1059-7794
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## Abstract The short sequence related to hepatitis C virus (HCV1) is detected by a labelβfree DNA hybridization biosensor. The sensor relies on the immobilization of a 20βmer oligonucleotide containing 2 guanine and 11 cytosine bases denoted PHCV1 as probe on the pencil graphite electrode (PGE). T