✦ LIBER ✦

Detection of parvovirus B19 DNA, antigen, and particles in the human fetus

✍ Scribed by Dr. J. P. Clewley; B. J. Cohen; Anne M. Field

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 594 KB
Volume: 23
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890230409

No coin nor oath required. For personal study only.

✦ Synopsis

Human parvovirus B 19 commonly infects children, causing erythema infectiosum (fifth disease). However, there is a significant adult population which has not been exposed to the virus and, consequently, does not have protective antibody. Recent reports have associated B19 infection during pregnancy with fetal death, although normal outcome of pregnancy is more common. To characterise further the role of B19 infection in fetal deaths, a series of laboratory investigations has been undertaken on tissues obtained at autopsy. These have demonstrated the presence of virion-sized DNA by Southern blotting, viral antigen by radioimmunoassay, and viral particles by electron microscopy, all from tissues of hydrops fetalis. These data confirm that the human parvovirus B19 can cross the placenta and replicate in fetal tissues.

📜 SIMILAR VOLUMES

Undenatured parvovirus B19 antigens are

Undenatured parvovirus B19 antigens are essential for the accurate detection of parvovirus B19 IgG

✍ Kerr, S.; O'Keeffe, G.; Kilty, C.; Doyle, S. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 239 KB 👁 2 views

Recombinant versions of parvovirus B19 capsid proteins VP1 and VP2 are used for immunodiagnostic assays for detection of antiviral antibodies. The immune response to B19 is characterized by a gradual loss of antibodies directed against linear epitopes of VP2. A similar occurrence for antibodies rais

Evidence for persistence of human parvov

Evidence for persistence of human parvovirus B19 DNA in bone marrow

✍ Cassinotti, P.; Burtonboy, G.; Fopp, M.; Siegl, G. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 35 KB 👁 2 views

A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The pre

B19 parvovirus induced fetal hydrops: ra

B19 parvovirus induced fetal hydrops: rapid and simple diagnosis by detection of B19 antigens in amniotic fluids

✍ G. Gentilomi; M. Zerbini; G. Gallinella; S. Venturoli; E. Manaresi; R. Morandi; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 63 KB 👁 2 views

In our study we describe the direct detection of parvovirus B19 capsid antigens in amniotic fluid samples for the rapid and simple prenatal diagnosis of B19 induced fetal hydrops. The assay was performed on amniotic fluid specimens from fetal hydrops dotted on nylon membranes. The two capsid antigen

Nested polymerase chain reaction assay f

Nested polymerase chain reaction assay for the detection of B19 parvovirus DNA in human immunodeficiency virus patients

✍ M. Musiani; A. Azzi; M. Zerbini; D. Gibellini; S. Venturoli; K. Zakrzewska; M. C 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 583 KB

## Abstract Persistent B19 parvovirus infection has been recognized in immunocompromised patients, often occurring with a low‐titer viremia. In this study, nested polymerase chain reaction (PCR) for the detection of B19 parvovirus DNA was carried out on the sera of 49 human immunodeficiency virus (

Case report: Electron microscopic detect

Case report: Electron microscopic detection of human parvovirus (B19) in a patient with HIV infection

✍ Dr. Ian L. Chrystie; June D. Almeida; Jan Welch 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 741 KB

## Abstract We report the findings on an HIV‐positive patient found to be infected with human parvovirus B19. In a comparative study of sequential sera, direct electron microscopy (EM) had the same sensitivity as DNA hydridisation for the detection of the virus. EM did not require specific reagents

Human parvovirus B19 infected fetal live

Human parvovirus B19 infected fetal liver as a source of antigen for a radioimmunoassay for B19 specific IgM in clinical samples

✍ Diana Westmoreland; Bernard J. Cohen 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 741 KB

A radioimmunoassay for human parvovirus B19 IgM was developed using virus antigen derived from infected fetal liver obtained post mortem. The specificity and sensitivity of this assay, compared with an established radioimmunoassay using serum antigen, was determined by testing 126 sera by both techn