Detection of oxidative species and low-molecular-weight DNA in skin following dermal exposure with JP-8 jet fuel

✍ Scribed by James V. Rogers; Palur G. Gunasekar; Carol M. Garrett; Mahendra B. Kabbur; James N. McDougal

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 128 KB
• Volume: 21
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.799

✦ Synopsis

Abstract

Dermal absorption of JP‐8 jet fuel can lead to skin irritation within hours after exposure. This study detected the formation of oxidative species and low‐molecular‐weight DNA in rat skin as potential indicators of JP‐8‐induced skin injury. At 0, 1, 2, 4 and 6 h after the beginning of a 1‐h exposure, skin samples were removed and analyzed for oxidative species formation and low‐molecular‐weight DNA analysis. At 1, 2 and 4 h, mean oxidative species levels increased significantly (P < 0.05) above unexposed samples. Significantly higher (P < 0.05) low‐molecular‐weight DNA values were observed at 4 and 6 h compared with unexposed controls. These results demonstrate significant increases in oxidative species and low‐molecular‐weight DNA levels in the skin following dermal exposure to JP‐8. These responses may serve as indicators of skin injury following exposure to JP‐8 jet fuel and other volatile chemicals or mixtures. Copyright © 2001 John Wiley & Sons, Ltd.