Dermal Exposure to m-Xylene Leads to Increasing Oxidative Species and Low Molecular Weight DNA Levels in Rat Skin

✍ Scribed by James V. Rogers; Palur G. Gunasekar; Carrol M. Garrett; James N. McDougal

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 189 KB
• Volume: 15
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/jbt.21

✦ Synopsis

Abstract

Dermal absorption of organic solvents, such as m‐xylene, can lead to skin inflammation and pathological changes
within hours after exposure. This study detected oxidative species formation and low molecular weight (LMW) DNA in rat
skin as potential indicators of m‐xylene‐induced skin injury. At 0, 1, 2, 4, and 6 h after the beginning
of a 1‐h exposure, skin samples were removed and analyzed for oxidative species formation and LMW DNA analysis. At 2 h, mean
oxidative species levels increased significantly (P < 0.05) above unexposed samples. Significantly higher
(P < 0.05) LMW DNA values were observed at 2, 4, and 6 h compared to unexposed controls. These results show
that oxidative species formation and LMW DNA levels in the skin may serve as indicators for predicting safe exposure levels to
m‐xylene and other volatile organic solvents. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol
15:228–230, 2001