Detection of N-nitroso-bile acids at 285 nm in reverse-phase HPLC

✍ Scribed by Yoshio Araki; Ken-ichi Mukaisyo; Hiroyuki Sugihara; Yoshihide Fujiyama; Takanori Hattori

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 802 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800230

✦ Synopsis

Abstract

In the present study, we developed a novel, simple, and specific detection method using an RP‐HPLC at UV 285 nm for the separation and quantification of N‐nitroso‐bile acids. First, we found that N‐nitroso‐bile acids have a specific spectrophotometric absorbance at 285 nm. Using this 285 nm detection system, we could especially measure N‐nitroso‐bile acids, even in co‐existence of non‐N‐nitroso‐bile acids. Next, we observed the decomposition of N‐nitroso‐glychocholate under alkaline, acidic, and neutral conditions. N‐nitroso‐glychocholate rapidly decomposed under alkaline conditions (pH 9) (t~1/2~ = 0.96 h), but remained fairly stable under acidic (pH 2) (t~1/2~ = 12.8 h) and neutral (pH 7) (t~1/2~ = 7.8 h) conditions. This study is the first report, which simply and specifically analyzes N‐nitroso‐bile acids using an RP‐HPLC system.