Trace Enrichment and HPLC Analysis of PAHs in Edible Oils and Fat Products, using Liquid Chromatography on Electron Acceptor Stationary Phases in Connection with Reverse Phase and Fluorescence Detection
✍ Scribed by Perrin, J. L. ;Poirot, N. ;Liska, P. ;Thienpont, A. ;Felix, G.
- Publisher
- John Wiley and Sons
- Year
- 1993
- Weight
- 531 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0931-5985
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✦ Synopsis
The determination of PAHs in lipids is beset with many difficulties, resulting from the low level of individual PAHs ( p g l k g ) , the complexity of extraction and clean-up procedures required to isolate the PAHs from the oil or the food components. The purpose of this report is to dercrihc a new method for multi-PAHs determination in lipidic matrixes. The clean-up step is realized by Donor Acceptor Complex Chromatography (DACC) with a tetrachlorophthalimidopropyl (TCPI) modified silica. Neutral lipids and other minor components, especially tocopherols which can interfere with PAH, are eluted with a hcx;inr/methyltertiobutylether mixture. Then, PAH are eluted with pure methylene chloride and analyzed by gradient reversed phase HPLC in combination with wavelength programmed fluorescence detection. This method allows an easy and fast quantitative recovery of aromatic compounds