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Detection of multiple gains and losses of genetic material in ten glioma cell lines by comparative genomic hybridization

✍ Scribed by Gayatry Mohapatra; Dong H. Kim; Burt G. Feuerstein

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 647 KB
Volume: 13
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870130203

No coin nor oath required. For personal study only.

✦ Synopsis

A protocol for comparative genomic hybridization by use of nucleotides directly labeled with fluorochromes was used to map regions of deletion and amplification in ten glioma cell lines. The protocol greatly reduced experimental artifacts. W e detected several genetic aberrations, including whole chromosome loss and gain, partial loss and gain, possible isochromosome, and higher level DNA amplification. The most frequent losses (in order of frequency) occurred on chromosomes I 0, 18, I 3, I I, 9, I4,4, 6. I. and X. The most common gain occurred on chromosome 7. Several sites of previously known and unknown amplifications , . were observed. Genes Chromosom Cancer 1386-93 (1995).

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