Patients with acute leukemia in clinical remission may still have up to 10 10 residual malignant cells (the upper limit of detection by standard morphologic techniques). Sensitive techniques to detect minimal residual disease (MRD) may allow better estimates of the leukemia burden and help the selec
Detection of minimal residual disease by mutant p53 immunocytochemistry in acute myelogenous leukemia
β Scribed by Kattamis, Antonios C.; Tsangaris, George Th.; Vamvoukakis, John; Moschovi, Maria; Grafakos, Stelios; Tzortzatou-Stathopoulou, Fotini
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 385 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0098-1532
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t(8;21) is one of the common chromosomal translocations in acute myelogenous leukemia (AML). Using a recently developed real-time quantitative polymerase chain reaction (PCR) system, we analyzed the minimal residual disease (MRD) in bone marrow samples from seven AML patients with t(8;21) at differe
Because previous PCR-based methodologies for detection of minimal residual disease (MRD) in leukemia patients have been too cumbersome to allow for widespread clinical usefulness, we have employed a real-time quantitative PCR (RQ-PCR) system to develop an MRD assay for t(12;21). We initially determi