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Detection of interspecific and intraspecific variation inPanicummillets through random amplified polymorphic DNA

✍ Scribed by H. K. M'Ribu; K. W. Hilu

Publisher
Springer
Year
1994
Tongue
English
Weight
870 KB
Volume
88-88
Category
Article
ISSN
0040-5752

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✦ Synopsis

The potential use of random amplified polymorphic DNA (RAPD) was evaluated as a source of genetic markers for studying variation among four species of Panicum and within the crop species P. miliaceum and P. sumatrense. Polymorphism in RAPD markers was observed across and within species. The four species were distinct in RAPD patterns and were separated at low correlation values even with small samples involving single genotypes per species. Accessions of P. miliaceum were grouped according to geographical regions of origin. The study demonstrated that unlike isozyme and protein electrophoresis patterns, RAPD markers can be applied to studying genetic diversity, defining gene pools, and identifying cultivars for this group of millets.

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