Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis

✍ Scribed by H. N. Asemota; J. Ramser; C. Lopéz-Peralta; K. Weising; G. Kahl

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 716 KB
• Volume: 92
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037118

✦ Synopsis

We have used random amplified polymorphic DNA (RAPD) analysis to characterize eleven cultivars of the five economically most important yam species grown in Jamaica (Dioscorea alata, D . cayenensis, D . rotundata, D . trifida and D . esculenta) . Amplification of genomic DNA samples with nine different arbitrary 10mer primers revealed a total of 338 different band positions, ranging in size from 0 .3 to 2 .5 kb . RAPD patterns proved to be highly reproducible and somatically stable . While no variation was observed among plants belonging to the same cultivar, a large number of intervarietal and interspecific polymorphisms enabled us to reliably discriminate between all Jamaican cultivars investigated .