Detection of interleukin and interleukin-receptor mRNA in human heart by polymerase chain reaction
β Scribed by Richard O. Han; Phillip E. Ray; Kenneth L. Baughman; Arthur M. Feldman
- Book ID
- 115764172
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 297 KB
- Volume
- 181
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Analysis of cytokine mRNA levels in interleukin-4-transgenic mice by quantitative polymerase chain reaction* Interleukin (IL)-4-transgenic mice were used as a model system to study the consequences of low levels of IL-4 expression for the expression of other cytokines examined by quantitative p
We describe a simplified and sensitive polymerase chain reaction (PCR)-based method for the quantification of low-abundance RNA for mouse cytokine receptor genes. Accurate quantification is achieved in a two-step protocol which uses a synthetic RNA as an internal standard. The proper titration of th