Detection of Immunoglobulin κ Light Chain Rearrangements by Polymerase Chain Reaction: An Improved Method for Detecting Clonal B-Cell Lymphoproliferative Disorders
✍ Scribed by Jerry Z. Gong; Sherman Zheng; Roberto Chiarle; Christine De Wolf-Peeters; Giorgio Palestro; Glauco Frizzera; Giorgio Inghirami
- Book ID
- 117022557
- Publisher
- American Society for Investigative Pathology
- Year
- 1999
- Tongue
- English
- Weight
- 188 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0002-9440
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Seminested polymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity-determining region 3 of the immunoglobulin (Ig) gene heavy chain from the malignant cell specimens of patients with leukemias and lymphomas of B-cell lineage. Two different pairs of primers were u