The sensitivities of dot blot hybridisation and in situ filter hybridisation for the detection of HPV DNA were compared. Dot blot hybridisation was 10-50 times more sensitive than in situ filter hybridisation in detecting HPV 16 DNA in the cervical cancer cell lines SiHa and CaSki. Cervical smears c
Detection of human papillomaviruses (HPV) 16 and 18 in cervical smears by in situ hybridisation
✍ Scribed by M. Eržen; J. Marin; M. Uršič-Vrščaj; A. Možina
- Book ID
- 118952722
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 82 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0301-2115
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## Abstract The polymerase chain reaction (PCR) was applied for the detection of human papilloma virus (HPV) infection, in samples obtained from the uterine cervices of 202 asymptomatic women with normal cytology in Northern Greece. About 41.8% of the women with microscopically and cytologically no
Persistent infection of the uterine cervix with high-risk human papillomaviruses (HPV) is causally associated with cancer of the cervix. A few studies have reported the presence of HPV DNA in the blood of women with cervical neoplasia. The aim of this study was to determine if HPV DNA could be detec
It has been recognized that human papillomavirus infection is the major causal factor for high-grade cervical lesions. The aim of the study was to evaluate the relationship between HPV 16 and 18 viral loads and cervical status in different age strata. A duplex real time PCR method was devised to det