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Evaluation of different DNA-DNA hybridisation techniques in detection of HPV 16 DNA in cervical smears and biopsies

✍ Scribed by Marion T. E. Cornelissen; Ko J. Van Der Velden; Jan M. M. Walboomers; Maarten A. Briët; Henk L. Smits; Jan Van Der Noordaa; Jan Ter Schegget

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
638 KB
Volume
25
Category
Article
ISSN
0146-6615

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✦ Synopsis

The sensitivities of dot blot hybridisation and in situ filter hybridisation for the detection of HPV DNA were compared. Dot blot hybridisation was 10-50 times more sensitive than in situ filter hybridisation in detecting HPV 16 DNA in the cervical cancer cell lines SiHa and CaSki. Cervical smears collected from 51 women with a history of one or more abnormal cervical smears were tested by both hybridisation techniques for the presence of HPV 16 DNA; 11 were positive in the in situ filter hybridisation, 35 in the dot blot hybridisation. Thirty-five cervical biopsies available from this group of 5 I women were processed for dot blot hybridisatiodn 30 of the 35 cases the results of this hybridisation corresponded with the results of the dot blot hybridisation on the smears.

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