Detection of human coronavirus 229E-specific antibodies using recombinant fusion proteins
β Scribed by A. Pohl-Koppe; T. Raabe; S.G. Siddell; V. ter Meulen
- Book ID
- 113256533
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 819 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0166-0934
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A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-strande
## To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by e