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Detection of human Coronavirus 229E in nasal specimens in large scale studies using an RT-PCR hybridization assay

โœ Scribed by Sophie Vallet; Arnaud Gagneur; Pierre J Talbot; Marie-Christine Legrand; Jacques Sizun; Bertrand Picard


Book ID
116735714
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
100 KB
Volume
18
Category
Article
ISSN
0890-8508

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Detection of human coronavirus 229E in n
โœ Dr.; Dr. S. Myint; S. Siddell; D. Tyrrell ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 480 KB

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-strande