Detection of herpes simplex virus DNA in a cutaneous squamous cell carcinoma by in situ hybridization
β Scribed by A. L. Claudy; M. C. Chignol; Y. Chardonnet
- Publisher
- Springer-Verlag
- Year
- 1989
- Tongue
- English
- Weight
- 840 KB
- Volume
- 281
- Category
- Article
- ISSN
- 0340-3696
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β¦ Synopsis
A case of a cutaneous squamous cell carcinoma (SCC) occurring at the site of a long-standing recurrent HSV infection is described. No deficit of the cell-mediated immunity was recorded. HSV2 was isolated in several viral cultures. HSV DNA was visualized in the SCC by in situ hybridization with biotinylated probes in paraffin-embedded tissue. The samples were negative for HPV2, 5, 16, and 18 probes. A causal relationship between HSV infection and cutaneous SCC is hypothesized.
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A diagnostic hybridization assay for detecting herpes simplex virus type 1 (HSV-1) in ocular specimens was developed using cloned viral DNA as a probe. This hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target DNA by a streptavidin/alkaline phosphatase sy
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