A sensitive and specific two-stage polymerase chain reaction (PCR) technique was developed for the simultaneous amplification and detection of specific genomic sequences of hepatitis B virus (HBV) and hepatitis C virus (HCV) in serum samples. Initially, HCV-RNA was reverse transcribed to cDNA. This
✦ LIBER ✦
Detection of hepatitis B virus sequences in serum by using in vitro enzymatic amplification
✍ Scribed by D. Larzul; F. Guigue; J.J. Sninsky; D.H. Mack; C. Bréchot; J.-L. Guesdon
- Book ID
- 119114644
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 709 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0166-0934
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For the detection of the HTLV-I provirus genomic DNA, we applied the polymerasechain-reaction (PCR) dot-blot assay system developed by Saiki et al. (14). DNA sequences coding the p24 in the gag region were chosen as targets to be amplified. We checked genomic DNAs extracted from peripheral blood lym