Detection of hepatitis B virus genome in hepatocellular carcinoma tissues with PCR-in situ hybridization
β Scribed by Biehuoy Shieh; Su-Er Lee; Yi-Chang Tsai; Ih-Jen Su; Ching Li
- Book ID
- 114140769
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 599 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0166-0934
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We have previously reported an analysis of DNA extracted from 31 primary liver tumors where, in 25 cases, we found chromosomal integration of hepatitis B virus DNA sequences. We describe here an investigation of the extent of the viral genome at each integration site in 15 of the hepatitis B virus D
## BACKGROUND. Although there have been many seroepideniiologic studies on hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) occurrence. the actual role of I ICV in hepatocarcinogenesis is unknown. ## METHODS. We have previously reported on a highly sensitive method of detectin
## Abstract A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using nonisoβtopic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive